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Mai 2018


Salle Amphi Pasteur

“ Endomembrane signaling and NLRP3 inflammasome activation ”


Dr. Roméo RICCI (Institut de génétique et de biologie moléculaire et cellulaireIllkirch-Graffenstaden)

contact : benedicte.py@inserm.fr

The inflammasome is a multiprotein complex that senses tissue damage and infectious agents to initiate immune responses. Distinct inflammasomes containing specific sensing molecules, including NLRP3, exist. We discovered that assembly of the NLRP3 inflammasome requires recruitment of NLRP3 to mitochondria-associated Endoplasmic Reticulum (ER) membranes (MAMs) adjacent to Golgi membranes. Compartmentalization of NLRP3 to MAMs is crucial for its self-oligomerization, while signaling from the Golgi is required to release NLRP3 from MAMs resulting in full assembly of the inflammasome. Our work thus provided unique insights into the spatially and temporally controlled mechanisms underlying NLRP3 inflammasome assembly.


Salle de SdT CRC

« Bilateral symmetry: from somites to brain »


Olivier POURQUIÉ  (Harvard Medical School)



Salle SdT CRC

« Is H3K4me3 Instructive for Transcription? Deconstructing the Relationship Between Chromatin and Gene Expression using Ultradian Metabolic Cycles. »


Jane MELLOR (University of Oxford)





Salle SdT CRC

“Dendritic Cell, Monocyte and Macrophage Biology: From Development to Functions”


Florent GINHOUX (Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR). Singapore)                     

contact : fl.cosset@ens-lyon.fr

Dendritic cells (DCs) are professional antigen-presenting cells that mediate immune responses. Important questions on the origins and differentiation paths of human DC populations remain unanswered. First, we combined two high-dimension techniques, single-cell mRNA sequencing and Cytometry by Time of Flight mass spectrometry to define and characterize human DC precursors (pre-DC) present in adult bone marrow and blood and revealed that pre-DC comprises distinct lineage-committed sub-populations. We also characterized the human fetal DC lineage and show that similar DC subpopulations can be identified in fetal tissues and are related to adult populations. However, fetal DC strongly promoted regulatory T-cell induction and inhibiting T-cell tumour-necrosis factor-α production through arginase-2 activity, indicating that they mediate homeostatic immune-suppressive responses during gestation. Altogether, we show that the DC lineage shares common development pathways from early fetal development to adulthood. However, DC functions differ drastically depending on the environment in which they are immersed.



Vendredi 25/05/2018

Salle SdT CRC


« Studying Nanoscale Chromatin Structure in living Multicellular Organism by quantitative FLIM-FRET Microscopy »







Salle de réunion IVPC

"High precision size & concentration measurement can have an incredible impact on your research of vesicles and viruses."
Camille ROESCH (www.izon.com)

contact : kathy.gallay@univ-lyon1.fr

We are at an exciting time when high precision measurement is easier and more accessible than ever. I would like to invite you to a seminar I am hosting, where you can learn how to apply this to your research. I will be covering TRPS measurement, which is the most advanced method of vesicle and virus analysis, providing highly accurate size, concentration and zeta potential measurements. The TRPS-based instrument, qNano Gold, allows you to normalize vesicle concentrations for functional analysis and to match vesicles isolated from different sources. This allows you to distinguish whether functional differences are caused by quantitative or compositional differences. Learn how you can rapidly analyze multiple populations, establishing the size distribution, physiological activity & zeta potential in my webinar. For better organization, please confirm your presence by completing the following survey :https://doodle.com/poll/iz6xbeimtx7csusw It will be possible to test 1 or 2 samples/person. If you want more details on the preparation of your sample, please send me an email camille.roesch@izon.com or give me a call on 0664636238

Jeudi 31/05/2018

Salle Sdt CRC


“Towards animal models for HCV vaccine research:,,Determinants of HCV species tropism”





Thomas PIETSCHMANN (TWINCORE - Zentrum für Experimentelle und Klinische Infektionsforschung GmbH. Hannover,Germany)                 

contact : fl.cosset@ens-lyon.fr

HCV infection affects ca. 71 million individuals worldwide. These patients are at risk to develop severe liver disease including cirrhosis and liver cancer. Novel therapies have been licensed and HCV infection can be cured. However, these therapies are expensive, many HCV infected patients are not diagnosed, and treatment-induced viral clearance does not protect from HCV re-infection. Therefore, development of a prophylactic vaccine is an important clinical need. Unfortunately, immunocompetent animal models are lacking so that analysis of protective immunity against HCV, and prioritization of vaccine candidates is difficult. HCV has a narrow species tropism and naturally infects only humans. The viral and host determinants governing HCV species tropism are incompletely defined. We used genome-wide cDNA screens and iterative virus selection and adaptation approaches to dissect viral and host factors that define the mechanisms of HCV species tropism. Using these approaches we identified two mouse factors  constitutively expressed in mouse livers that restrict HCV infection. Moreover, viral variants with extended species tropism were developed and characterized. Ultimately, this work should facilitate development of animal models for HCV vaccine research.



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