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IVPC- Vesicular traffic and tissular physiopathology GROUP

Presentation

 

image 1 IVPC FA group   Retroviruses exploit cellular machineries to propagate. In the last decade, impressive similarities have been observed in the generation and dissemination in the host cells of retroviruses and small cellular vesicles known as exosomes (Izquierdo-Useros, Puertas et al. 2011, Meckes and Raab-Traub 2011). These cellular vesicles are thought to facilitate intercellular communication processes and mediate immune functions (Simons and Raposo 2009). They are issued from internal endosome-derived membranes and are secreted by cells under normal or pathological condition (infection, cancer). Ranging from 30 to 150 nm in size, exosomes can incorporate proteins, microRNA (miRNA), mRNA, mtDNA, DNA and retrotransposons; they can mask viruses/viral components from immune surveillance, and transfer cargo between cells. They can contribute to the horizontal transfer of oncogenes (Al-Nedawi, Meehan et al. 2008, Demory Beckler, Higginbotham et al. 2013) or pathogenic elements such as virus elements (Ramakrishnaiah, Thumann et al. 2013, Nour and Modis 2014) or prion (Fevrier, Vilette, Archer et al. 2004), inducing deregulation of the recipient cell and /or propagation of the disease.

image2 IVPC FA group

 

Our research focus on the transfer of viral elements (oncogenic viral protein, RNA) via microvesicles from infected/transformed cells to naïve cells, and their impact on the cellular environment (immune regulation, cell transformation).  The JSRV retrovirus being an oncogenic virus, we study the capacity of the oncogenic viral protein to be transferred to naïve cells via microvesicles and/or exosomes and to modulate neighboring cells behavior. Having a long experience in lung epithelial cells models, we question the impact of exosomes/microvesicles on bronchioli and alveolar cells homeostasis, in the context of infection, and investigate their involvement in lung pathology development and maintenance.

The obviously broad biological and medical implications of microvesicles make them a significant and exciting area of research with potential high impact on our understanding of pathogenesis within the infected individual. Therefore understanding the interplay between viruses and microvesicles may contribute to the development of novel therapeutics or vaccines to control viral infections and prevent development of pathologies. 

 

 

Contact

 

  1. Responsable d'équipe : Fabienne  ARCHER, CR1 INRA, HDR

    Adresse :UMR 754 INRA-UCBL-EPHE

    50, Avenue Tony Garnier

    69366 Lyon Cedex 07

    Téléphone : 04 37 28 76 06

    Fax : 04 37 28 76 05

    E-mail :fabienne.archer@univ-lyon1.fr

 

 

Publications

 

1: Vituret C, Gallay K, Confort MP, Ftaich N, Matei CI, Archer F, Ronfort C, Mornex JF, Chanson M, Di Pietro A, Boulanger P, Hong SS. Transfer of the Cystic Fibrosis Transmembrane Conductance Regulator to Human Cystic Fibrosis Cells Mediated by Extracellular Vesicles. Hum Gene Ther. 2016 Feb;27(2):166-83.

2: Chebib N, Khouatra C, Lazor R, Archer F, Leroux C, Gamondes D, Thivolet-Bejui  F, Cordier JF, Cottin V. [Pulmonary lymphangioleiomyomatosis: From pathogenesis to management]. Rev Mal Respir. 2016 Oct;33(8):718-734.

3: Monot M, Archer F, Gomes M, Mornex JF, Leroux C. Advances in the study of transmissible respiratory tumours in small ruminants. Vet Microbiol. 2015 Dec 14;181(1-2):170-7.

4: Monot M, Erny A, Gineys B, Desloire S, Dolmazon C, Aublin-Gex A, Lotteau V, Archer F, Leroux C. Early Steps of Jaagsiekte Sheep Retrovirus-Mediated Cell Transformation Involve the Interaction between Env and the RALBP1 Cellular Protein. J Virol. 2015 Aug;89(16):8462-73.

5: Bolfa P, Nolf M, Cadoré JL, Catoi C, Archer F, Dolmazon C, Mornex JF, Leroux C. Interstitial lung disease associated with Equine Infectious Anemia Virus infection in horses. Vet Res. 2013 Dec 1;44:113.

6: Archer F, Abi-Rizk A, Desloire S, Dolmazon C, Gineys B, Guiguen F, Cottin V, Mornex JF, Leroux C. Lung progenitors from lambs can differentiate into specialized alveolar or bronchiolar epithelial cells. BMC Vet Res. 2013 Nov 8;9:224.

7: Viginier B, Dolmazon C, Lantier I, Lantier F, Archer F, Leroux C, Terzian C. Copy number variation and differential expression of a protective endogenous retrovirus in sheep. PLoS One. 2012;7(7):e41965

8: Cottin V, Archer F, Leroux C, Mornex JF, Cordier JF. Milestones in lymphangioleiomyomatosis research. Eur Respir Rev. 2011 Mar;20(119):3-6

9: Cottin V, Archer F, Khouatra C, Lazor R, Cordier JF. [Lymphangioleiomyomatosis]. Presse Med. 2010 Jan;39(1):116-25

10: Varela M, Golder M, Archer F, de las Heras M, Leroux C, Palmarini M. A large  animal model to evaluate the effects of Hsp90 inhibitors for the treatment of lung adenocarcinoma. Virology. 2008 Feb 5;371(1):206-15.

11: Leroux C, Girard N, Cottin V, Greenland T, Mornex JF, Archer F. Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep. Vet Res. 2007 Mar-Apr;38(2):211-28.

12: Archer F, Jacquier E, Lyon M, Chastang J, Cottin V, Mornex JF, Leroux C. Alveolar type II cells isolated from pulmonary adenocarcinoma: a model for JSRV expression in vitro. Am J Respir Cell Mol Biol. 2007 May;36(5):534-40.

13: Suau F, Cottin V, Archer F, Croze S, Chastang J, Cordier G, Thivolet-Béjui F, Mornex JF, Leroux C. Telomerase activation in a model of lung adenocarcinoma. Eur Respir J. 2006 Jun;27(6):1175-82.

14: Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G. Cells release prions in association with exosomes. Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9683-8.

16: Beringue V, Vilette D, Mallinson G, Archer F, Kaisar M, Tayebi M, Jackson GS, Clarke AR, Laude H, Collinge J, Hawke S. PrPSc binding antibodies are potent inhibitors of prion replication in cell lines. J Biol Chem. 2004 Sep 17;279(38):39671-6.

17: Archer F, Bachelin C, Andreoletti O, Besnard N, Perrot G, Langevin C, Le Dur A, Vilette D, Baron-Van Evercooren A, Vilotte JL, Laude H. Cultured peripheral neuroglial cells are highly permissive to sheep prion infection. J Virol. 2004 Jan;78(1):482-90.

18: Laude H, Vilette D, Le Dur A, Archer F, Soulier S, Besnard N, Essalmani R, Vilotte JL. New in vivo and ex vivo models for the experimental study of sheep scrapie: development and perspectives. C R Biol. 2002 Jan;325(1):49-57.

19: Vilette D, Andreoletti O, Archer F, Madelaine MF, Vilotte JL, Lehmann S, Laude H. Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):4055-9. 

20: Finn JT, Weil M, Archer F, Siman R, Srinivasan A, Raff MC. Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases. J Neurosci. 2000 Feb 15;20(4):1333-41. 

21: Archer FR, Doherty P, Collins D, Bolsover SR. CAMs and FGF cause a local submembrane calcium signal promoting axon outgrowth without a rise in bulk calcium concentration. Eur J Neurosci. 1999 Oct;11(10):3565-73.

22: Bolsover S, Ashworth R, Archer F. Activator of calcium influx proves a slippery customer. J Physiol. 1999 May 15;517 ( Pt 1):2.