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Protein Characterization Unit

 

Purpose

The Protein Characterization Unit specializes in proteomics and intermolecular interaction analysis by surface plasmon resonance (SPR) and bio-layer interferometry (BLI).

  • Proteomic :

-Identification of proteins after enzymatic digestion by coupling nanoLC-MS/MS

-Relative quantification of proteins

-characterization of post-translational modfications

-Molecular mass measurement

  • BLItz to detect and quantify proteins and characterize biomolecular interactions
  • Biacore T200 to study biomolecular interactions by surface plasmon resonance (SPR).

 

How to cite SFR Biosciences in publications

 

Services

Proteome and biomolecular interaction analyses.

► MASS SPECTROMETRY

Additional information :

► ANALYSES OF BIOMOLECULAR INTERACTIONS by INTERFEROMETRY (BLItz system) and by Surface Plasmon Resonance (BIACORE T200 system)

For information  :

Equipment

  • 3 Mass spectrometers :
    1. Q Exactive HF Biopharma (Thermo Scientific) : nanoelectrospray source, quadripole-orbitrap, HCD fragmentation, nanoRSLC coupling (Thermo Scientific)
    2. VOYAGER De Pro (Sciex) : MALDI-TOF
    3. LTQ Velos (Thermo Scientific) :  nanoelectrospray source, double trap analyser, fragmentations CID/ETD, nanoLC NCP U3000 (Thermo Scientific)
  • 1 BLITZ (Pall ForteBio) for interferometry analyses
  • 1 Biacore T200 (GE Healthcare) - SPR.
  1.  

TRAINING

 

User fees

Information on user fees is available on intranet (équipes contributrices et équipes académiques) or upon demand.

 

Documentation

  1. Fiche échantillon à remplir pour la spectrométrie de masse.
  2. Biacore T100 Instrument Handbook
  3. Brochure Biacore T200
  4. BiacoreT200 System Data File
  5. Biacore Consommables Brochure et Catalogue

 

Links

  1. Réservation du Biacore (pour les utilisateurs déclarés – accès sécurisé)
  2. Réservation du BLITZ (pour les utilisateurs déclarés – accès sécurisé)
  3. How to find us  (intranet)

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