Gilles LEMAÎTRE  (ISTEM Corbeil-Essonnes- institut des cellules souches pour le traitement et l’étude des maladies monogéniques)      

Invité par: Jérôme Lamartine

IBCP Confernce Room level -1                                                                                                            

Serge BIRMAN (ESPCI, Paris)

Andrew BARLOW (Thermo fisher scientific) 

Contact: Bénédicte Py et Christophe Chamot (benedicte.py@inserm.fr)

Automated microscopy and multi-parameter image processing developed this last decade made possible high content screening (HCS) with image-based read-outs. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameter of interest by automated image analysis. In particular, HCS is extensively used in gene invalidation-based screens (mutant, siRNA, CRISPR/Cas9) and small compounds screens, and can be instrumental in a tremendous variety of research fields relying on image-based read-outs (cell viability, proliferation, apoptosis, autophagy, endocytosis, mitochondria health, cell and organelle morphology, inflammasome).
Several teams of the SFR Biosciences have expressed a strong interest in getting access to this technology on our campus. Andrew Barlow (Thermo fisher scientific) will give a seminar to the SFR Biosciences community to introduce the potential of HCS approach and discuss the available options of the Thermo Fisher devices (CellInsight CX5, CX7 and ArrayScan) including the number of illumination channels, possibility of confocal, available objectives, focus system, upgrade for live cell imaging.            

In the afternoon, Andrew Barlow will be available for discussion. Please contact Bénédicte Py (benedicte.py@inserm.fr)

Amphi K, ENS

Muriel COCAIGN-BOUSQUET  (LISBP - INSA de Toulouse)

Bacteria adapt their growth and metabolism continuously to the environment changes. The regulation networks are complex with multi-scale regulations at the transcriptional but also post-transcriptional level. Compared to transcription, the rules and regulations of mRNA degradation are not well understood. I will show with Omics approaches, how the rate of mRNA degradation can change significantly in cells sensing environmental changes and how mRNA degradation is coordinated to transcription process.

Martine BIARD-PIECHACZYK (CPBS FRE3689 CNRS Université de Montpellier)     

Hôte: M. Dreux. Marlene.dreux@inserm.fr

http://www.cpbs.cnrs.fr/spip.php?rubrique9&lang=fr

Programmation scientifique C. CHAGNEAU, F. LAURENT, S. PICO, F. VANDENESCH

S'inscrire avant le 09/12/2016                                                                        

IFE, 19 allée de Fontenay -69007 Lyon

 Hector ESCRIVA  (Banyuls Marine Laboratory)   

 Salle des Thèses Chantal Rabourdin-Combe