Inscription gratuite mais obligatoire (lien inscription.) Cette journée aura lieu sur le campus de la DOUA, amphithéâtre de l’IPNL de 9H à 16H (buffet sur place) avec la participation de :
* Dr Daniel SAGE, EPFL-Lausanne, Suisse « Directional Image Analysis - Applications to bioimaging »
* Dr Andrea BASSI, Politechnico Milano, Italie « In toto imaging with light sheet microscopy »
* Dr Corinne LORENZO, ITAV- Toulouse « Spatio-temporal dynamics of the bystander effects on cellular and tissular levels in an integrated model: a study with light sheet microscopy » * Dr Jean-Baptiste SIBARITA, IINS, Bordeaux « Deciphering synaptic molecule organization and dynamics using quantitative single-molecule localization microscopy »
* Dr. Françoise PEYRIN, ESRF, Grenoble « 3D imaging of the ultrastructure of bones by nano CT Xray phase imaging »
* Dr. Guy COURBEBAISSE, CREATIS, Lyon « Characterization of platelet aggregates by holographic microscope for modeling thrombus formation within brain aneurysms »

campus de la DOUA, amphi IPNL

Paul BOURGINE

 Invité par B. Verrier et F. Mallein-Gerin

_{Bone is a complex organ offering structural and mechanical support of our body, but also consisting in our hematopoietic center. Both the regeneration of the skeletal (e.g. fracture healing) and hematopoietic (e.g. leukemia) tissues are of major clinical concern, requiring the development of innovative tools to study the Bone compartments, and ultimately design regenerative therapies. During my talk, I will describe the exploitation of two recent and complementary biotechnological platforms, offering the engineering of bone and bone marrow models (in vitro approach), and the ectopic reconstitution of human bone marrow environments in mouse (in vivo approach). The two systems display a high degree of modularity for adaptation to specific scenarios (e.g. generation of niches with customized molecular signature, study in pathologic conditions), and can be exploited in a synergistic fashion. This will allow gaining considerable basic biology knowledge in a humanized set-up of higher translational relevance. Clinical implications include the generation of bone grafts substitute, the expansion/differentiation of human blood cells for clinical purposes, and the screening of drugs for blood cancer treatment.Bone is a complex organ offering structural and mechanical support of our body, but also consisting in our hematopoietic center. Both the regeneration of the skeletal (e.g. fracture healing) and hematopoietic (e.g. leukemia) tissues are of major clinical concern, requiring the development of innovative tools to study the Bone compartments, and ultimately design regenerative therapies. During my talk, I will describe the exploitation of two recent and complementary biotechnological platforms, offering the engineering of bone and bone marrow models (in vitro approach), and the ectopic reconstitution of human bone marrow environments in mouse (in vivo approach). The two systems display a high degree of modularity for adaptation to specific scenarios (e.g. generation of niches with customized molecular signature, study in pathologic conditions), and can be exploited in a synergistic fashion. This will allow gaining considerable basic biology knowledge in a humanized set-up of higher translational relevance. Clinical implications include the generation of bone grafts substitute, the expansion/differentiation of human blood cells for clinical purposes, and the screening of drugs for blood cancer treatment.}

salle de conférence IBCP

Le colloque IMMUNINV 2017 se tient cette année à Lyon sur le Campus de la Doua, du 7 au 9 juin 2017. Ces journées rassemblent la communauté des chercheurs s’intéressant à l’immunologie des Invertébrés, afin d’aborder un ensemble de thématiques qui touche aux mécanismes de l’immunité et aux interactions entre les invertébrés, leurs symbiotes - pathogènes ou mutualistes - et l’environnement. Les travaux de recherche concernent à la fois des organismes modèles et des invertébrés d’intérêt agronomique (insectes ravageurs), aquacole (espèces élevées) ou épidémiologique (vecteurs de maladies).

Tous les renseignements sont disponibles sur le site web du colloque : https://immuninv2017.sciencesconf.org/

Andrea DIEPOLD - Max Planck Institute for Terrestrial Microbiology, Marburg, Germany

invited by C. Lesterlin

_{The type III secretion system (T3SS) is one of the most elegant and efficient methods that bacteria have evolved to manipulate host cells by direct protein injection. While the structure of the parts of the type III secretion “injectisome” has been resolved to a molecular level, the molecular details of protein export and regulation remain unclear. To understand how the T3SS works on the molecular level, and how it is regulated, I study the system in action by analyzing functional fluorescently labelled T3SS components in live bacteria. We have previously shown that a soluble component of the T3SS, the cytosolic C-ring, exchanges between the injectisome and a cytosolic pool. This exchange correlates with effector export through the T3SS.
But what happens within the cytosol? Combining biochemical interaction studies and fluorescence microscopy in live bacteria, we uncovered a dynamic network of interactions that is directly influenced by external cues, revealing a potential new way to regulate T3SS activity.}

Salle de conférences IBCP

_{L'emploi des moyennes en physiologie et en médecine ne donne le plus souvent qu'une fausse précision aux résultats en détruisant le caractère biologique des phénomènes. » Claude Bernard
This quote from the father of modern physiology and medicine highlights the need for measuring biological phenomena at their relevant scale. Current techniques now allow interrogating molecular contents at the single cell level, unleashing the full power of the cell theory formulated in 1839.
This single-cell day of the SFR biosciences will be dedicated to expose how old questions can obtain new answers using single-cell approaches.
We will listen to two keynotes speakers: Jeff Mold (Karolinska Institute - Sweden) and Gilad Yaakov (Weizmann Institute of Science - Israel).
We also will hear flash presentations from the SFR platforms, and talks by young scientists from each different laboratory.}

Domaine St Joseph

Danijela VIGNEVIC - Institut Curie, Paris

Salle des thèses Chantal Rabourdin-Combe

 Patrick McGRATH

hôte : Marie Delattre

Salle des thèses Chantal Rabourdin-Combe

Vincent VILLERET Unité de glycobiologie structurale et fonctionnelle, Université de Lille

invited by JM Jault

_{The outer membrane (OM) of Gram-negative bacteria is a protective layer for the cell against noxious chemicals such as antibiotics and other anti-bacterial molecules, but also a major barrier for the translocation of macromolecules. It is thus a site of intense trafficking both to import nutrient and to export macromolecules. Protein secretion is critical for pathogens to adhere to, invade or intoxicate host tissues. The two-partner secretion (TPS) pathway exports large proteins for colonization, biofilm formation and bacterial warfare. The TpsB transporters, which secrete their extracellular TpsA 'substrates' are transmembrane beta-barrel proteins. They are part of the ubiquitous superfamily of Omp85 transporters, found in the membranes of bacteria and of various organelles of endosymbiotic origin, where they mediate protein insertion into or translocation across membranes. In the superfamily are the BamA transporters that assemble proteins into the bacterial OM. The transport of their substrates by TpsB and BamA transporters is coupled to substrate folding into beta-helices and beta-barrels, respectively. One of the best-studied TPS systems comprises the translocase FhaC and its substrate filamentous hemagglutinin (FHA), which is functionally involved in virulence and biofilm formation in Bordetella pertussis, the whooping-cough agent. Crystallographic structure determination of FhaC has provided the first depiction of the general Omp85 architecture, revealing clues into how FhaC acts to secrete the FHA virulence protein. In addition, recent structural and functional studies have provided}

Salle de Conférence IBCP

 Chloé FERRAL - IRCAN INSERM U1081 CNRS UMR7284, Université de Nice  

Hôte : S. Pentalacci

Salle des thèses Chantal Rabourdin-Combe

Pr. Anne-Sophie KORGANOW - Université de Strabsourg, UPR 3572-IBMC, Strasbourg

contact : jean-françois.nicolas@chu-lyon.fr
alexandre.belot@chu-lyon.fr

Amphji Pasteur - Tour Inserm CERVI