Octobre 2023
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Lundi 02/10/2023 M6 Meeting room (RDC) Séminaire Externe
Lundi 02/10/2023 Salle 107 ENS-L Séminaire Externe |
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« tbc » Annapurna DEVI ALLU (Indian Institute of Science Education and Research (IISER) Host : Teva Vernoux
« The iRhom-ADAM17 complex as a central regulator of inflammatory and growth factor signalling » Stefan DÜSTERHÖFT (University Hospital Aachen/ RWTH Aachen) Hôte : Christiane Riedel
Membrane-anchored signalling mediators such as cytokines (e.g. TNFα) and many EGF receptor ligands (e.g. TGFa) as well as adhesion molecules are proteolytically released (shed) by the metalloproteinase ADAM17. Thus, ADAM17 plays a key role in many inflammatory and developmental processes. In addition, ADAM17 is an important step in the entry of viruses such as SARS-CoV and pestiviruses into cells. iRhom1 and iRhom2 are inactive members of the rhomboid protease family that are essential for the transport of ADAM17 to the cell surface as well as its regulation. However, the structure-function determinants and molecular regulation of the iRhom-ADAM17 complex are poorly understood. We are using bioinformatics (including A.I.-based structure modelling), molecular and cell biological as well as in vivo approaches to gain insight into the molecular mechanisms governing the functions and activity of the iRhom-ADAM17 complex. We have identified distinct regions in iRhom that are differentially responsible for iRhom functions and ADAM17 regulation, including binding, protein stability and protease activity. These mechanistic insights will form the basis for the development of methods to modify ADAM17 activity, which may be used to design novel therapeutic strategies against ADAM17-associated pathologies such as chronic inflammation and cancer.
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Lundi 02/10/2023 Salle SDT CRC Séminaire Externe |
" Comparative hijacking by alphaviruses - a viral centric approach for more relevant drug target discovery? " | |
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Dr. Guillaume CARISSIMO
(heads the host-pathogen interactions lab in A*STAR ID Labs. Singapore)
Hôte : Andrés Pizzorno
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Mercredi 04/10/2023 Amphi B Séminaire Externe |
« Exploring the cellular basis of ovule curvature using 3D digital ovules » | |
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Kay SCHNEITZ (Technical University Munich) Host : Charlie Scutt | |
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Vendredi 06/10/2023 09h00 Salle conf IBCP Séminaire Externe
Vendredi 06/10/2023 11h00 Salle SDT CRC Séminaire Externe
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« Studying poxvirus infection using cellular cryo electron tomography » Emmanuelle QUEMIN(Institute for Integrative Biology of the Cell (I2BC)) --- Hôte : Christiane Riedel Poxviruses are widely distributed large and enveloped dsDNA viruses. Historically, Variola virus caused smallpox epidemics before its eradication after the first international vaccination campaign with Vaccinia virus (VACV). The recent multi-country outbreak of Monkeypox virus stressed that zoonotic poxviruses can still spread in human populations. Over the years, VACV has become a powerful model to study poxviruses and investigate key aspects of pox-biology that remain unknown.
« Coordination of different microtubule networks to position a nucleus asymmetrically » Antoine GUICHET (Institut Jacques Monod Paris) --- Hote : Equipe Grammont
The accurate control of the position of the nucleus is essential for many cellular contexts. The forces applied to positioned the nucleus vary according to cell type, but all originate from elements of the cytoskeleton such as the microtubules.
They can generate forces directly through polymerization or by the involvement of molecular motors. An important issue is to understand how different microtubule cues can collaborate to accurately position the nucleus.
The Drosophila oocyte is a large polarized cell in which the nucleus is asymmetrically position. This positioning is mandatory for the polarity axis establishment of the future embryo.
It presents an ideal system to study how different microtubule networks coordinate to generate the forces necessary for positioning the nucleus.
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Lundi 09/10/2023 Salle SDT CRC Séminaire Externe
Lundi 09/10/2023 Salle SDT CRC Séminaire Externe |
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« A structural account of bunyavirus membrane fusion »
Jan HELLERT (Centre for Structural Systems Biology (CSSB), Leibniz Institute of Virology (LIV)) -- Hôte : Christiane Riedel
"Living in a changing world – The genomics of adaptation to environmental variation and experimental selection for larval malnutrition. "
Martin KAPUN -- Hôte : Adbou Khila
I will present genome-wide analyses of clinal genomic variation in the fruit-fly D. melanogaster densely sampled through time and space on different continents where we tested for associations between genetic and environmental variation. These analyses reveal complex demographic patterns and pronounced clinal genetic variation along different geographical and environmental transects, which are predominantly shaped by chromosomal inversions. Besides, we found evidence for strong clinal selection on genes involved in metabolic processes. In the second part of my talk I will therefore present the results of a long-term selection experiment, where flies were selected for larval malnutrition. We found evidence that candidate genes are associated with metabolic pathways and that most SNP that are putative targets of selection represent standing genetic variation commonly found in natural populations.
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Mardi 10/10/2023 Amphi Pasteur Séminaire Externe
Mardi 10/10/2023 Salle Condorcet
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« A lethal genome collective : brain tropism acquisition by measles virus » Pr. Roberto CATTANEO (Professor of Biochemistry and Molecular Biology Mayo Clinic – Rochester – Minnesota - USA) -- Hôte : Mathieu Mateo
It is increasingly appreciated that pathogens can spread as collective infectious units. Brain autopsy material from a patient who died with subacute sclerosing panencephalitis (SSPE) provided a unique opportunity to reconstruct the evolution of an RNA genome collective in a human brain. Multiple distinct genome lineages co-replicated even at the level of single cells. Driver mutations were independently and recurrently selected across brain regions and genetic backgrounds. Specific mutations were constrained to intermediate prevalence by frequency-dependent selection, which modulated fusogenicity. Analyses of this and other SSPE brains indicate that plastically evolving genome collectives are key for measles virus brain colonization and raise profound questions about the importance of genome collectives in human disease more broadly.
CHROMATIN CLUB Organizers : Béatrice Horard & Armelle Corpet Contact : armelle.corpet@univ-lyon1,fr Program
14h00-15h00 Regulation of gene dosage during early mouse development
Katia Ancelin (Institut Curie, Paris)
15h00-15h30 What is the direct impact of R-loops on the genome ?
Vincent Vanoosthuyse (LBMC, Lyon)
15h30-16h00 Coffee break / meet the speakers
16h00-16h30 Nucleolar reorganization after cellular stress is orchestrated by SMN shuttling
between nuclear compartments
Ambra Mari (INMG, Lyon)
16h30-17h00 An original epigenetic signature for primordial germ cells in chicken
Clémence Kress (SBRI, Lyon)
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Mercredi 11/10/2023 M6 Meeting room (rdc) Séminaire Externe
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« Importunate lover goes to DEAD-End: A cell fusion changes destination of second sperm cell pair and avoids polyspermy » | |
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Daisuke MARUYAMA (Yokohama University, Japan)
Host : Thomas WIDIEZ
Synergid cells are versatile gate keepers of fertilization in flowering plants. They attract pollen tubes, facilitate pollen tube discharge, and exert in pollen tube reception. In the canonical pollen tube reception, released sperm cells immediately and precisely reach to boundary between the egg and central cell by using mechanical guide of receptive synergid cell. In this seminar, I will show a novel pollen tube reception pathway, DEAD-End. In the DEAD-End, sperm cells finally go to the endosperm through synergid cell. This switching of sperm cell destination depends on non-gametic cell fusion that takes place between persistent synergid and early endosperm (synergid-endosperm fusion, SE fusion). DEAD-End only occurred when fertilize ovules accidentally receive second pollen tube. I propose that DEAD-End is a novel polyspermy block mechanism keeping extra sperm cells away from fertilization-competent zone in developing ovule.
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Mardi 17/10/2023 Salle SDT CRC Séminaire Externe
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« tbc » | |
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Petra CIFROVÁ (Charles University, Prague)
Host : Yoan Coudert
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Mardi 24/10/2023 ? Séminaire Externe
Mardi 24/10/2023 Salle SDT CRC Forum CIRI
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" tbc "
James CASTELLI-GAIR HOMBRIA (Centro Andaluz de Biologia del Desarrollo, Sevilla, Spain) -- Hôte : Julie Carnesecchi
« In vivo models of Epstein Barr virus induced lymphomagenesis and cell-mediated immune control »
Pr. Christian MÜNZ (Viral Immunobiology Institute of Experimental Immunology, University of Zürich) --- Hôte : Henri Gruffat
The Epstein Barr virus (EBV) is a ubiquitous human tumor virus causing around 2% of all malignancies in humans. Despite its remarkable growth transforming ability in vitro, our immune system protects the vast majority of adult virus carriers from EBV associated tumors. Mice with reconstituted human immune system components have been explored by us and others as an in vivo model for EBV infection, lymphomagenesis and immune control. These allowed us to investigate how co-infections with the Kaposi sarcoma associated herpesvirus (KSHV) and the human immunodeficiency virus (HIV) modify EBV associated malignancies and their immune control. Furthermore, we were able to characterize the non-redundant role of lymphocyte receptors and of lymphocyte differentiation stages during EBV specific immune control. Our studies provide further insights into the composition of the highly efficient immune protection against EBV associated lymphomas that most virus carriers establish and that EBV specific vaccination should elicit.
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Jeudi 26/10/2023 Salle SDT CRC Séminaire Externe
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" The hidden power of the 3'UTR in gene expression regulation " | |||
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Alexandra MOREIRA (Gene Regulation, i3S and ICBAS, University of Porto, Portugal)
Hôte : Julie Carnesecchi
Most human genes go through alternative polyadenylation in different physiological conditions, producing multiple mRNAs with important implications in health and disease. The 3’ untranslated region (3’UTR) of the mRNA, frequently defined by alternative polyadenylation, contains multiple regulatory sequences representing a main hub for gene expression regulation. This regulatory mechanism is partly dictated by RNA-binding proteins (RBPs) that define not only the site of cleavage and polyadenylation of the nascent transcript, but also determine the fate of the mRNA by modulating its stability, transport, subcellular localization, translation and protein localization.
I will present the work my group has been developed on the molecular mechanisms involved in gene expression regulation by alternative polyadenylation in the 3’UTRs and their physiological function, in different model systems. We use a multidisciplinary approach that combines advanced 3’RNA-Seq methodologies and bioinformatics with well-established methods in cellular and molecular biology. I will also present how we are applying this fundamental knowledge to develop new therapeutic tools. |
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Vendredi 27/10/2023 Salle SDT CRC Séminaire Externe
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« tbc »
Nitin PHADNIS (University of Utah, Salt Lake City, USA)
Hote : Benjamin Loppin
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