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AniRA - PBES

https://www.sfr-biosciences.fr/plateformes/animal-sciences/AniRA-PBES/AniRA%20-%20PBES/leadImage

Purpose

The AniRA-PBES core facility (Plateau de Biologie Expérimentale de la Souris - Experimental Biology in Mice) provides researchers with transgenic murine lines (establishment, breeding and production), as well as genotyping and phenotyping services. The facility has protected areas for experiments that require BSL1, BSL2 and BSL3 containment.

 

AniRA-PBES is part of the regional AniRA technological core facility that is certified by the national IBiSA label (IBiSA coordinates core facilities in the area of life sciences throughout France), and is a member of Celphedia (a French National Research Infrastructure for animal models).

As such, AniRA-PBES is open to academic and industrial scientists all over France.

 

AniRA-PBES trains researchers in animal experimentation (regulatory courses for project designers), transgenesis, embryo handling, cryopreservation of murine transgenic lines and stem cell culture.

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Services

  • To breed and establish SPF (specific pathogen free) mice (3000 ventilated cages).
  • To supply and house mice in experimental facilities that require BSL1, BSL2 or BSL3 containment (2000 cages)
  • To perform various types of investigations (sampling, injections, weighing…)
  • To establish genetically modified mouse strains by traditional transgenesis, homologous recombination (stem cells), lentiviral transgenesis (in collaboration with the vectorology core facility), genomic engineering (TALE nuclease and gene editing)
  • To produce SPF mice by embryo transfer
  • To cryopreserve and distribute mouse strains (embryo, sperm and ovaries)
  • To perform genotype analysis by PCR (done by AniRA-AGCThe facility is certified ISO 9001 :2015.)
  • To run xMAP assays (Multi-Analyte Profiling, Luminex Corp)
  • To perform phenotype analyses of the immune and bone systems of knock-out and transgenic mice (AniRA-ImmOs)
  • To provide the PHARE software, specifically designed for managing animal facilities (breeding and housing of the animals, as well as for billing customers).

 

Prior to their use, all experimental protocols must be approved by the Ethics Committee (CECCAPP) and/or the Transgenesis Committee.

 

Contact/Localisation

How to acceed (en intranet)

Plateau de Biologie Expérimentale de la Souris (PBES)

  1. ENS de Lyon
  2. 46 Allée d’Italie
  3. 69364 Lyon Cedex 07

 

  1. PBES : pbes@ens-lyon.fr
  2. PHARE : phare@ens-lyon.fr
  3.  

Training

  • Regulatory courses for project designers, Level B: "Conventional and transgenic animal models for biological and medical research – Animal Facility Technology”, Inter-University Degree (D.I.U.), École Normale Supérieure de Lyon/Université Claude Bernard Lyon I - 1 session per year, in June, Plaquette de formation (expérimentation animale 2020)

 

  • One-to-one tutoring in stem cell culture, transgenesis, lentigenesis, embryo handling, cryopreservation and rederivation of murine strains.

 

  • Training on the use of the Phare software, specifically designed for managing animal facilities.

For information on the software, please contact phare@ens-lyon.fr.

 

 

User fees

Information on user fees is available on: ask us

Tarifs accessibles en intranet (équipes contributrices et équipes académiques) .

 

Documentation

  1. Manuel Qualité du PBES
  2. Plaquette de formation expérimentation animale 2020
  3. Règlement intérieur (version 2014)
  4. Organigramme du PBES (version 2014)
  5. Procédures d’entrées de nouvelles lignées
  6. Fiche introduction nouvelle lignée
  7. Formulaire de transfert de souris
  8. Formulaire  de transfert de souris vers la zone A2

 

LiNKS

  1. CECCAPP (informations sur l’expérimentation animale et l’éthique)
  2. AFSTAL
  3. Ecole Nationale Vétérinaire de Lyon
  4. Office Vétérinaire Suisse
  5. Conseil Canadien de Protection des Animaux
  6. PHARE
  7. Un guide des risques biologiques (CNRS)
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