2021
Stiffness measurement is a biomarker of skin ageing in vivo.
-
Runel G, Cario M, Lopez-Ramirez N, Malbouyres M, Ruggiero F, Bernard L, Puisieux A, Caramel J, Chlasta J, Masse
- .Exp Dermatol. 2020 Dec;29(12):1233-1237
- Acknowledged platform: PRECI
Abstract :Human skin is particularly vulnerable to age-related deterioration and undergoes profound structural and functional changes, reflected in the external skin appearance. Skin ageing is characterized by features such as wrinkling or loss of elasticity. Even if research advances have been done concerning the molecular mechanisms that underlie these changes, very few studies have been conducted concerning the structure stiffness of the skin organ as a whole. In this study, we showed, thanks to human skin reconstructs and the Japanese Medaka fish model, that biomechanics is a new biomarker of skin ageing. We revealed that global stiffness measurement by Atomic Force Microscopy, since modulated through ageing in these models, can be a new biomarker of skin ageing, and reflects the profound reorganization of the dermis extracellular matrix, as shown by Transmission Electron Microscopy. Moreover, our data unveiled that the Japanese Medaka fish could represent a highly relevant integrated model to study skin ageing in vivo.
DOI : 10.1111/exd.14195
Pubmed ID :32967047
A Scaffold- and Serum-Free Method to Mimic Human Stable Cartilage Validated by Secretome
Isis Côrtes, Renata A M Matsui, Mayra S Azevedo, Anderson Beatrici, Kleber L A Souza, Guilaume Launay, Frédéric Delolme, José M Granjeiro, Catherine Moali, Leandra S Baptista
Tissue Eng Part A, . 2021 Mar;27(5-6):311-327.
Acknowledged platform: PSF
Abstract :A stabilized cartilage construct without signs of hypertrophy in chondrocytes is still a challenge. Suspensions of adipose stem/stromal cells (ASCs) and cartilage progenitor cells (CPCs) were seeded into micromolded nonadhesive hydrogel to produce spheroids (scaffold- and serum-free method) characterized by size, immunohistochemistry, fusion, and biomechanical properties. After cell dissociation, they were characterized for mesenchymal cell surface markers, cell viability, and quantitative real-time polymerase chain reaction. Both targeted and nontargeted (shotgun mass spectrometry) analyses were conducted on the culture supernatants. Induced ASC spheroids (ø = 350 μm) showed high cell viability and CD73 downregulation contrasting to CD90. The transforming growth factor (TGF)-β3/TGF-β1 ratio and SOX9 increased (p < 0.05), whereas interleukin (IL)-6, IL-8, RUNX2, and ALPL decreased. Induced ASC spheroids were able to completely fuse and showed a higher force required to compression at day 14 (p < 0.0001). Strong collagen type II in situ was associated with gradual decrease of collagen type X and a lower COLXA1 gene expression at day 14 compared with day 7 (p = 0.0352). The comparison of the secretome content of induced and non-induced ASCs and CPCs identified 138 proteins directly relevant to chondrogenesis of 704 proteins in total. Although collagen X was absent, thrombospondin-1 (TSP-1), described as antiangiogenic and antihypertrophic, and cartilage oligomeric matrix protein (COMP), a biomarker of chondrogenesis, were upregulated in induced ASC spheroids. Our scaffold- and serum-free method mimics stable cartilage acting as a tool for biomarker discovery and for regenerative medicine protocols. Impact Statement Promising adult stem cell sources for cartilage regeneration include adipose stem/stromal cells (ASCs) from subcutaneous adipose tissue. Our main objective was the development of a reproducible and easy-to-handle scaffold- and serum-free method to obtain stable cartilage from induced ASC spheroids. In addition to targeted protein profiling and biomechanical analysis, we provide the first characterization of the secretome composition for ASC spheroids, providing a useful tool to monitor in vitro chondrogenesis and a noninvasive quality control of tissue-engineered constructs. Furthermore, our secretome analysis revealed a potential novel biomarker-thrombospondin-1 (TSP-1), known by its antiangiogenic properties and recently described as an antihypertrophic protein.
DOI : 10.1089/ten.TEA.2018.0311
Pubmed ID :30734654
Improved cell signalling analysis by biofunctionalized nanospheres and imaging flow cytometry
Alice Koenig, Xavier Charmetant, Antoine Sicard, Sébastien Dussurgey, Olivier Thaunat
Cytometry Part A
Acknowledged platform: AniRA Cytométrie
Abstract :
The analysis of immune cell signaling is critical for the understanding of the biology and pathology of the immune system, and thus a mandatory step for the development of efficient biomarkers and targeted therapies. Phosflow, which has progressively replaced the traditional western blot approach, relies on flow cytometry to analyze various signaling pathways at a single-cell level. This technique however suffers a lack of sensitivity largely due to the low signal/noise ratio that characterizes cell signaling analysis. In this study, we describe a new technique, which combines the use of biofunctionalized nanospheres (i.e., synthetic particulate antigens, SPAg) to stimulate the immune cells in suspension and imaging flow cytometry to identify homogenously-stimulated cells and quantify the activity of the chosen signaling pathway in selected subcellular regions of interest. Using BCR signaling as model, we demonstrate that SIBERIAN (SPAg-assIsted suB-cEllulaR sIgnaling ANalysis) allows assessing immune cell signaling with unprecedented sensitivity and specificity.
DOI : 10.1002/cyto.a.24354
Pubmed ID :33866668
Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model.
Eyenga P., Roussel D., Rey B., Ndille P., Teulier L., Eyenga F., Romestaing C., Morel J., Gueguen-Chaignon V., Sheu S-S.
Intensive Care Med Exp. 2021,9:19
Acknowledged platform: PSF
Abstract :
Background: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis.
Methods: A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 h after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the proinflammatory interleukin-1β (IL-1β) in diaphragms.
Results: CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome c oxidase activity.
Conclusion: In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function.
DOI : 10.1186/s40635-021-00384-w
Pubmed ID :33825987
Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein
Published by Oxford University Press on behalf of Nucleic Acids Research.
Acknowledged platform: PLATIM AniRA Cytométrie
Abstract :
Epstein-Barr virus (EBV) is a human herpesvirus associated with human cancers worldwide. Ex vivo, the virus efficiently infects resting human B lymphocytes and induces their continuous proliferation. This process is accompanied by a global reprogramming of cellular gene transcription. However, very little is known on the impact of EBV infection on the regulation of alternative splicing, a pivotal mechanism that plays an essential role in cell fate determination and is often deregulated in cancer. In this study, we have developed a systematic time-resolved analysis of cellular mRNA splice variant expression during EBV infection of resting B lymphocytes. Our results reveal that major modifications of alternative splice variant expression appear as early as day 1 post-infection and suggest that splicing regulation provides-besides transcription-an additional mechanism of gene expression regulation at the onset of B cell activation and proliferation. We also report a role for the viral proteins, EBNA2 and EBNA-LP, in the modulation of specific alternative splicing events and reveal a previously unknown function for EBNA-LP-together with the RBM4 splicing factor-in the alternative splicing regulation of two important modulators of cell proliferation and apoptosis respectively, NUMB and BCL-X.
DOI : 10.1093/nar/gkab787
Pubmed ID :34530456
Improved cell signaling analysis by biofunctionalized nanospheres and imaging flow cytometry
2021 International Society for Advancement of Cytometry.
Acknowledged platform: AniRA Cytométrie
Abstract :
The analysis of immune cell signaling is critical for the understanding of the biology and pathology of the immune system, and thus a mandatory step for the development of efficient biomarkers and targeted therapies. Phosflow, which has progressively replaced the traditional western blot approach, relies on flow cytometry to analyze various signaling pathways at a single-cell level. This technique however suffers a lack of sensitivity largely due to the low signal/noise ratio that characterizes cell signaling analysis. In this study, we describe a new technique, which combines the use of biofunctionalized nanospheres (i.e., synthetic particulate antigens, SPAg) to stimulate the immune cells in suspension and imaging flow cytometry to identify homogenously-stimulated cells and quantify the activity of the chosen signaling pathway in selected subcellular regions of interest. Using BCR signaling as model, we demonstrate that SIBERIAN (SPAg-assIsted suB-cEllulaR sIgnaling ANalysis) allows assessing immune cell signaling with unprecedented sensitivity and specificity.
DOI : 10.1002/cyto.a.24354
Pubmed ID :33866668
Specific detection of memory T‐cells in COVID‐19 patients using standardized whole‐blood Interferon gammarelease assay
Acknowledged platform: AniRA Cytométrie
Abstract :
Antigen-specific T-cells are essential for protective immunity against SARS-CoV-2. We set up a semi-automated whole-blood Interferon-gamma release assay (WB IGRA) to monitor the T-cell response after stimulation with SARS-CoV-2 peptide pools. We report that the WB IGRA is complementary to serological assays to assess SARS-CoV-2 immunity.
DOI : 10.1002/eji.202149296
Pubmed ID :34387859
Immunogenicity and efficacy of heterologous ChadOx1/BNT162b2 vaccination
Acknowledged platform: AniRA Cytométrie
Abstract :
Following severe adverse reactions to the AstraZeneca ChAdOx1-S-nCoV-19 vaccine1,2, European health authorities have recommended that patients under the age of 55 who received one dose of ChAdOx1-S-nCoV-19 vaccine receive a second dose of Pfizer BNT162b2 vaccine as a booster. However, the effectiveness and the immunogenicity of this vaccination regimen have not been formally tested. Here, we show that the heterologous ChAdOx1-S-nCoV-19/BNT162b2 combination confers better protection against Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection than the homologous BNT162b2/BNT162b2 combination in a real-world observational study of healthcare workers (n=13121). To understand the underlying mechanism, we conducted a longitudinal survey of the anti-spike immunity conferred by each vaccine combination. Both combinations induced strong anti-spike antibody (Ab) responses but sera from heterologous vaccinated individuals displayed a stronger neutralizing activity, regardless of the SARS-CoV-2 variant. This enhanced neutralizing potential was correlated with increased frequencies of switched and activated memory B cells recognizing the SARS-CoV-2 Receptor Binding Domain (RBD). The ChAdOx1-S-nCoV-19 vaccine induced a weaker IgG response but a stronger T cell response than the BNT162b2 vaccine after the priming dose, which could explain the complementarity of both vaccines when used in combination. The heterologous vaccination regimen could therefore be particularly suitable for immune compromised individuals.
DOI : 10.1038/s41586-021-04120-y
Pubmed ID :34673755
A longitudinal study of SARS-CoV-2-infected patients reveals a high correlation between neutralizing antibodies and COVID-19 severity
Acknowledged platform: AniRA Cytométrie
Abstract :
Understanding the immune responses elicited by SARS-CoV-2 infection is critical in terms of protection against reinfection and, thus, for public health policy and vaccine development for COVID-19. In this study, using either live SARS-CoV-2 particles or retroviruses pseudotyped with the SARS-CoV-2 S viral surface protein (Spike), we studied the neutralizing antibody (nAb) response in serum samples from a cohort of 140 SARS-CoV-2 qPCR-confirmed infections, including patients with mild symptoms and also more severe forms, including those that required intensive care. We show that nAb titers correlated strongly with disease severity and with anti-spike IgG levels. Indeed, patients from intensive care units exhibited high nAb titers; conversely, patients with milder disease symptoms had heterogeneous nAb titers, and asymptomatic or exclusive outpatient-care patients had no or low nAbs. We found that nAb activity in SARS-CoV-2-infected patients displayed a relatively rapid decline after recovery compared to individuals infected with other coronaviruses. Moreover, we found an absence of cross-neutralization between endemic coronaviruses and SARS-CoV-2, indicating that previous infection by human coronaviruses may not generate protective nAbs against SARS-CoV-2. Finally, we found that the D614G mutation in the spike protein, which has recently been identified as the current major variant in Europe, does not allow neutralization escape. Altogether, our results contribute to our understanding of the immune correlates of SARS-CoV-2-induced disease, and rapid evaluation of the role of the humoral response in the pathogenesis of SARS-CoV-2 is warranted.
DOI : 10.1038/s41423-020-00588-2
Pubmed ID :33408342
Sequential actions of EOMES and T-BET promote stepwise maturation of natural killer cells
Acknowledged platform: AniRA Cytométrie
Abstract :
EOMES and T-BET are related T-box transcription factors that control natural killer (NK) cell development. Here we demonstrate that EOMES and T-BET regulate largely distinct gene sets during this process. EOMES is dominantly expressed in immature NK cells and drives early lineage specification by inducing hallmark receptors and functions. By contrast, T-BET is dominant in mature NK cells, where it induces responsiveness to IL-12 and represses the cell cycle, likely through transcriptional repressors. Regardless, many genes with distinct functions are co-regulated by the two transcription factors. By generating two gene-modified mice facilitating chromatin immunoprecipitation of endogenous EOMES and T-BET, we show a strong overlap in their DNA binding targets, as well as extensive epigenetic changes during NK cell differentiation. Our data thus suggest that EOMES and T-BET may distinctly govern, via differential expression and co-factors recruitment, NK cell maturation by inserting partially overlapping epigenetic regulations.
DOI : 10.1038/s41467-021-25758-2
Pubmed ID :34521844
NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly
Acknowledged platform: AniRA Cytométrie
Abstract :
NLRP3 controls the secretion of inflammatory cytokines IL-1β/18 and pyroptosis by assembling the inflammasome. Upon coordinated priming and activation stimuli, NLRP3 recruits NEK7 within hetero-oligomers that nucleate ASC and caspase-1 filaments, but the apical molecular mechanisms underlying inflammasome assembly remain elusive. Here we show that NEK7 recruitment to NLRP3 is controlled by the phosphorylation status of NLRP3 S803 located within the interaction surface, in which NLRP3 S803 is phosphorylated upon priming and later dephosphorylated upon activation. Phosphomimetic substitutions of S803 abolish NEK7 recruitment and inflammasome activity in macrophages in vitro and in vivo. In addition, NLRP3-NEK7 binding is also essential for NLRP3 deubiquitination by BRCC3 and subsequently inflammasome assembly, with NLRP3 phosphomimetic mutants showing enhanced ubiquitination and degradation than wildtype NLRP3. Finally, we identify CSNK1A1 as the kinase targeting NLRP3 S803. Our findings thus reveal NLRP3 S803 phosphorylation status as a druggable apical molecular mechanism controlling inflammasome assembly.
DOI : 10.1038/s41467-021-26142-w
Pubmed ID :34615873
A single-chain and fast-responding Light-Inducible Cre recombinase as a novel optogenetic switch
Acknowledged platform: AniRA Cytométrie
Abstract :
Optogenetics enables genome manipulations with high spatiotemporal resolution, opening exciting possibilities for fundamental and applied biological research. Here, we report the development of LiCre, a novel light-inducible Cre recombinase. LiCre is made of a single flavin-containing protein comprising the AsLOV2 photoreceptor domain of Avena sativa fused to a Cre variant carrying destabilizing mutations in its N-terminal and C-terminal domains. LiCre can be activated within minutes of illumination with blue light without the need of additional chemicals. When compared to existing photoactivatable Cre recombinases based on two split units, LiCre displayed faster and stronger activation by light as well as a lower residual activity in the dark. LiCre was efficient both in yeast, where it allowed us to control the production of β-carotene with light, and human cells. Given its simplicity and performances, LiCre is particularly suited for fundamental and biomedical research, as well as for controlling industrial bioprocesses.
DOI : 10.1038/s41467-021-26142-w
Pubmed ID :34615873
Genetic in vivo engineering of human T lymphocytes in mouse models
NATURE PROTOCOLS | 12 April 2021 |
Acknowledged platform: AniRA Cytométrie
Abstract :
Receptor targeting of vector particles is a key technology to enable cell type-specific in vivo gene delivery. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them to express chimeric antigen receptors (CARs). Here, we provide detailed protocols for the generation of CD4- and CD8-targeted LVs (which takes ~9 d in total). We also describe how to humanize immunodeficient mice with hematopoietic stem cells (which takes 12-16 weeks) and precondition (over 5 d) and administer the vector stocks. Conversion of the targeted cell type is monitored by PCR and flow cytometry of blood samples. A few weeks after administration, ~10% of the targeted T-cell subtype can be expected to have converted to CAR T cells. By closely following the protocol, sufficient vector stock for the genetic manipulation of 10-15 humanized mice is obtained. We also discuss how the protocol can be easily adapted to use LVs targeted to other types of receptors and/or for delivery of other genes of interest.
DOI : 10.1038/s41596-021-00510-8
Pubmed ID :33846629
Functional Heterogeneity of Mammalian IFITM Proteins against HIV-1
JOURNAL OF VIROLOGY 25 August 2021
Acknowledged platform: AniRA Cytométrie
Abstract :
Interferon-induced transmembrane proteins (IFITMs) are a family of interferon-inducible proteins that inhibit a broad range of viruses by interfering with viral-to-cellular membrane fusion. The antiviral activity of IFITMs is highly regulated by several posttranslational modifications and by a number of protein domains that modulate steady-state protein levels, trafficking, and antiviral effectiveness. Taking advantage of the natural diversity existing among IFITMs of different animal species, we have compared 21 IFITMs for their ability to inhibit HIV-1 at two steps, during virus entry into cells (target cell protection) and during the production of novel virion particles (negative imprinting of virion particles' infectivity). We found a high functional heterogeneity among IFITM homologs with respect to both antiviral modalities, with IFITM members that exhibit enhanced viral inhibition, while others have no ability to block HIV-1. These differences could not be ascribed to known regulatory domains and could only be partially explained through differential protein stability, implying the existence of additional mechanisms. Through the use of chimeras between active and inactive IFITMs, we demonstrate that the cross talk between distinct domains of IFITMs is an important contributor of their antiviral potency. Finally, we identified murine IFITMs as natural variants competent for target cell protection, but not for negative imprinting of virion particles' infectivity, suggesting that the two properties may, at least in principle, be uncoupled. Overall, our results shed new light on the complex relationship between IFITMs and viral infection and point to the cross talk between IFITM domains as a novel layer of regulation of their activity. IMPORTANCE IFITMs are broad viral inhibitors capable of interfering with both early and late phases of the replicative cycle of many different viruses. By comparing 21 IFITM proteins issued from different animal species for their ability to inhibit HIV-1, we have identified several that exhibit either enhanced or impaired antiviral behavior. This functional diversity is not driven by differences in known domains and can only be partly explained through differential protein stability. Chimeras between active and inactive IFITMs point to the cross talk between individual IFITM domains as important for optimal antiviral activity. Finally, we show that murine IFITMs are not capable of decreasing the infectivity of newly produced HIV-1 virion particles, although they retain target cell protection abilities, suggesting that these properties may be, in principle, disconnected. Overall, our results shed new light on the complex layers of regulation of IFITM proteins and enrich our current understanding of these broad antiviral factors.
DOI : 10.1128/JVI.00439-21
Pubmed ID :34160255
The Inflammasome Adaptor ASC Delays UV-Induced Skin Tumorigenesis in Beta HPV38 E6 and E7 Transgenic Mice
JOURNAL OF INVESTIGATIVE DERMATOLOGY Janvier 2021
Acknowledged platform: AniRA Cytométrie
Abstract :
DOI : 10.1016/j.jid.2020.04.028
Pubmed ID :32470340
A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity
COMMUNICATIONS BIOLOGY FEB 16 2021
Acknowledged platform: AniRA Cytométrie
Abstract :
During the cancerous transformation of normal hepatocytes into hepatocellular carcinoma (HCC), the enzyme catalyzing the first rate-limiting step of glycolysis, namely the glucokinase (GCK), is replaced by the higher affinity isoenzyme, hexokinase 2 (HK2). Here, we show that in HCC tumors the highest expression level of HK2 is inversely correlated to GCK expression, and is associated to poor prognosis for patient survival. To further explore functional consequences of the GCK-to-HK2 isoenzyme switch occurring during carcinogenesis, HK2 was knocked-out in the HCC cell line Huh7 and replaced by GCK, to generate the Huh7-GCK+/HK2- cell line. HK2 knockdown and GCK expression rewired central carbon metabolism, stimulated mitochondrial respiration and restored essential metabolic functions of normal hepatocytes such as lipogenesis, VLDL secretion, glycogen storage. It also reactivated innate immune responses and sensitivity to natural killer cells, showing that consequences of the HK switch extend beyond metabolic reprogramming.
DOI : 10.1038/s42003-021-01749-3
Pubmed ID :33594203
A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity
COMMUNICATIONS BIOLOGY FEB 16 2021
Acknowledged platform: AniRA Cytométrie
Abstract :
During the cancerous transformation of normal hepatocytes into hepatocellular carcinoma (HCC), the enzyme catalyzing the first rate-limiting step of glycolysis, namely the glucokinase (GCK), is replaced by the higher affinity isoenzyme, hexokinase 2 (HK2). Here, we show that in HCC tumors the highest expression level of HK2 is inversely correlated to GCK expression, and is associated to poor prognosis for patient survival. To further explore functional consequences of the GCK-to-HK2 isoenzyme switch occurring during carcinogenesis, HK2 was knocked-out in the HCC cell line Huh7 and replaced by GCK, to generate the Huh7-GCK+/HK2- cell line. HK2 knockdown and GCK expression rewired central carbon metabolism, stimulated mitochondrial respiration and restored essential metabolic functions of normal hepatocytes such as lipogenesis, VLDL secretion, glycogen storage. It also reactivated innate immune responses and sensitivity to natural killer cells, showing that consequences of the HK switch extend beyond metabolic reprogramming.
DOI : 10.1038/s42003-021-01749-3
Pubmed ID :33594203
Activation of cGAS/STING pathway upon paramyxovirus infection
ISCIENCE jun 25 2021
Acknowledged platform: AniRA Cytométrie
Abstract :
During inflammatory diseases, cancer, and infection, the cGAS/STING pathway is known to recognize foreign or self-DNA in the cytosol and activate an innate immune response. Here, we report that negative-strand RNA paramyxoviruses, Nipah virus (NiV), and measles virus (MeV), can also trigger the cGAS/STING axis. Although mice deficient for MyD88, TRIF, and MAVS still moderately control NiV infection when compared with wild-type mice, additional STING deficiency resulted in 100% lethality, suggesting synergistic roles of these pathways in host protection. Moreover, deletion of cGAS or STING resulted in decreased type I interferon production with enhanced paramyxoviral infection in both human and murine cells. Finally, the phosphorylation and ubiquitination of STING, observed during viral infections, confirmed the activation of cGAS/STING pathway by NiV and MeV. Our data suggest that cGAS/STING activation is critical in controlling paramyxovirus infection and possibly represents attractive targets to develop countermeasures against severe disease induced by these pathogens.
DOI : 10.1016/j.isci.2021.102519
Pubmed ID :34142033
PLA2R1 promotes DNA damage and inhibits spontaneous tumor formation during aging
Co-auteur Sophia : Huna et Al
Cell Detah Dis Febuary 16 2021
Acknowledged platform: PRECI
Abstract :
DOI : 10.1038/s41419-021-03468-3.
Pubmed ID :
Calcium channel ITPR2 and mitochondria-ER contacts promote cellular senescence and aging.
Nature Comm FEB 1 2021
Acknowledged platform: PRECI
Abstract :
DOI : 10.1038/s41467-021-20993-z.
Pubmed ID :
Staphylococcus aureus internalization impairs osteoblastic activity and early differentiation process
Scientific Report SEPT 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1038/s41598-021-97246-y
Pubmed ID :34480054
Specific detection of memory T-cells in COVID-19 patients using standardized whole-blood Interferon gammarelease assay
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1002/eji.202149296
Pubmed ID : 34387859
OVX836 Heptameric Nucleoprotein Vaccine Generates Lung Tissue-Resident Memory CD8+ T-Cells for Cross-Protection Against Influenza
Judith Del Campo, Julien Bouley, Marion Chevandier, Carine Rousset, Marjorie Haller, Alice Indalecio, Delphine Guyon-Gellin, Alexandre Le Vert , Fergal Hill , Sophia Djebali, Yann Leverrier, Jacqueline Marvel, Béhazine Combadière, Florence Nicolas
Frontiers in Immunology JUN 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.3389/fimmu.2021.678483
Pubmed ID : 34177921
Polyclonal expansion of TCR Vbeta 21.3+ CD4 + and CD8 + T cells is a hallmark of Multisystem Inflammatory Syndrome in Children
Science Immunology MAY 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1126/sciimmunol.abh1516
Pubmed ID :34035116
Staphylococcus aureus internalization impairs osteoblastic activity and early differentiation process
Scientific Report SEP 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1038/s41598-021-97246-y
Pubmed ID : 34480054
Wild versus lab house mice: Effects of age, diet, and genetics on molar geometry and topography
Journal of Anatomy AUG 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1111/joa.13529
Pubmed ID : 34333769
3D models related to the publication: Patterns of bilateral asymmetry and allometry in Late Devonian Polygnathus conodonts
Morpho-museum MAR 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.18563/journal.m3.126
Pubmed ID :
A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity
Laure Perrin-Cocon, Pierre-Olivier Vidalain, Clémence Jacquemin, Anne Aublin-Gex, Keedrian Olmstead, Baptiste Panthu, Gilles Jeans Philippe Rautureau, Patrice André, Piotr Nyczka, Marc-Thorsten Hütt, Nivea Amoedo, Rodrigue Rossignol, Fabian Volker Filipp, Vincent Lotteau, Olivier Diaz
Communications Biology FEB 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1038/s42003-021-01749-3.
Pubmed ID : 33594203
Patterns of bilateral asymmetry and allometry in Late Devonian Polygnathus conodonts
Palaeontology NOV 2021
Acknowledged platform: AniRA ImmOs
Abstract :
DOI : 10.1111/pala.12513
Pubmed ID :
The SARS-CoV-2 envelope and membrane proteins modulate maturation and retention of the spike protein, allowing assembly of virus-like particles
Biol Chem Jan-Jun 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1074/jbc.RA120.016175
Pubmed ID :33229438
Lipid anchoring and electrostatic interactions target NOT-LIKE-DAD to pollen endo-plasma membrane.
Cell Biol Oct 4 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1083/jcb.202010077
Pubmed ID : 34323919
Inducible depletion of PI(4,5)P 2 by the synthetic iDePP system in Arabidopsis
Nat Plants MAY 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1038/s41477-021-00907-z
Pubmed ID :34007035
Tissue folding at the organ-meristem boundary results in nuclear compression and chromatin compaction
Fal K, Korsbo N, Alonso-Serra J, Teles J, Liu M, Refahi Y, Chabouté ME, Jönsson H, Hamant O.
Proc Natl Acad Sci U S A. FEB 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1073/pnas.2017859118
Pubmed ID : 33608459
A nanodomain-anchored scaffolding complex is required for the function and localization of phosphatidylinositol 4-kinase alpha in plants
Plant Cell. May 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1093/plcell/koab135
Pubmed ID :34010411
A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process
Elife. Jun 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.7554/eLife.64507
Pubmed ID :34190687
Hybrid multimodal contrast agent for multiscale in vivo investigation of neuroinflammation
Nanoscale. Feb2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1039/d0nr07026b
Pubmed ID : 33555278
Imaging the living plant cell: from probes to quantification
Plant Cell Sep 2021
Acknowledged platform: PLATIM
Abstract :
DOI : 10.1093/plcell/koab237
Pubmed ID : 34586412