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Mars 2017

06/03 / 2017
« Cell fate decisions in a yeast MAPK pathway »

Pr Serge PELET

(Université de Lausanne,CH)       Hôte : G Yvert -

Serge Pelet is a leader in tracking the dynamics of cell responses to external stimuli. His lab developed very sensitive tools to catch molecular responses with high temporal precision. This is in yeast (of course) but the tools can in principle be exploited readily in other organisms. The cell-to-cell diversity of the response dynamics is also remarkable and informative.

06 / 03 / 2017
“Deciphering antifungal drug resistance mechanisms in fungal pathogens”  



Dominique SANGLARD
(CHUV Institute of Microbiology, Lausanne, CH)                                                 invited by P. Falson



07 / 03 / 2017
« A journey into biofabrication technologies »


Pr. Lorenzo MORONI (MERLN Institute for Technology-Inspired Regenerative Medicine
Maastricht university


 A key factor in scaffold-based tissue and organ regeneration relies on enhancing (stem) cell-material interactions to obtain the same original functionality. Different approaches include delivery of biological factors and surface topography modifications. Although both strategies have proved to augment cell activity on biomaterials, they are still characterized by limited control in space and time, which hampers the proper regeneration of complex tissues. Here, we present a few examples where the integration of biofabrication technology platforms allowed the generation of a new library of 3D scaffolds with tailored biological, physical, and chemical cues at the macro, micro, and nano scale. By engineering their topological properties, these porous biomaterials influence the activity of seeded cells, thereby initiating the regeneration of skeletal, vascular, and neural tissues. Future efforts should aim at further improving our understanding of scaffold topological properties to achieve a fine control on cell fate at multiple scales. This will enable the regeneration of complex tissues including vasculature and innervation, which will result in enhanced in vivo integration with surrounding tissues. By doing so, the gap from tissue to organ regeneration will be reduced, bringing regenerative medicine technologies closer to the clinics.

08 / 03 / 2017
" The Foxo3 / eomes axis controls CD4 T cell polarization "



         Anne DEJAN    (Inserm UMR1043/CNRS5282, Toulouse)  Invited by thierry.walzer@inserm.fr         

The overarching objective of our team is to dissect the cellular and molecular mechanisms underlying susceptibility to immune-mediated disease with a particular focus on inflammatory diseases of the central nervous system.
We recently demonstrated that the transcription factor Foxo3 plays a key role in the physiopathology of neuroinflammation since Foxo3-deficiency is associated with a significant decrease in the severity of EAE. We also provided evidence that this decreased severity of EAE is the consequence of the inability of Foxo3-deficient CD4 T cells to differentiate into pathogenic T helper-1 cells producing IFN-g and GM-CSF. At the molecular level, we identified Eomes as a direct target gene for Foxo3 in CD4+ T cells and we have shown that lentiviral-based overexpression of Eomes in Foxo3-deficient CD4+ T cells restored both IFN-g and GM-CSF production. Thus, the Foxo3-Eomes pathway is central to achieve the complete specialized gene program required for pathogenic Th1 cell differentiation and development of neuroinflammation.

  Amphithéâtre Pasteur                                            

13 /03 / 2017
«Regeneration versus embryonic development: molecular dynamics and scaling issues»



(Karolinska Inst. Stockholm)

Salle Chantal Rabourdin Combe

17 / 03 / 2017
« Improving scientific writing skills »



Julian VENABLES (Science et sens – Paris) Invited by : Didier Auboeuf

It is of paramount importance in modern science to sell your message in the most effective manner possible and this is a major challenge even for English-first-language scientists. In this seminar and workshop I will cover many aspects of clear concise scientific paper writing to inform the experienced scientist and student alike and help you communicate your ideas more effectively. I will discuss all aspects, from assembling and structuring manuscript sections to scientific style and grammar.
The abstract and title may be the last thing you write when you write a paper. The writing process is about creating and organising and the abstract should contain everything of paramount importance in your paper for conveying one simple message.

Julian VENABLES is an independent scientific editor and former English university genetics professor with over 20 first author publications and considerable grant and paper writing experience. He now concentrates on helping non-English first-language scientists get maximum recognition for their work, either individually or through seminars and workshops.



Salle Chantal Rabourdin-Combe

17 / 03 / 2017
« The power of CRISPR-Cas9 arrayed screen using synthetic crRNA libraries »

Amanda HAAS (GE Healthcare Life Sciences, Dharmacon)

CRISPR-Cas9 is a powerful tool for loss-of-function analysis, and to date has been adopted for large-scale functional screening primarily in the form of pooled sgRNA libraries. However, pooled screening is limited to enrichment or depletion phenotypic assays, thereby reducing the breadth and depth of biological questions that can be applied. An arrayed platform, which supports one-gene-per-well knockout, greatly expands the types of phenotypic assays to morphological and other high-content readouts, including multiparametric analysis. The experimental considerations for optimization and successful execution of an arrayed CRISPR-Cas9 screen using a two-part (dual) synthetic guide RNA system will be briefly described. We will present results from a multiparametric HCA screen using an arrayed crRNA library of cell cycle-related genes in a G1S cell cycle phase reporter cell line. This cell line expresses EGFP fused to a subcellular localization domain of Helicase B, which translocates between the nucleus and cytoplasm based on its phosphorylation state, driven by the cell cycle phase. Finally, we will review two recent publications using arrayed synthetic crRNAs and the utility of this approach.








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