LYMIC-PLATIM - Imaging and Microscopy Core Facility
Purpose
The Imaging and Microscopy Core Facility (PLATIM) provides to scientific community, a large offer of optical microscopy: conventional and fully automated video-microscopy, confocal and multi-photon and super resolution techniques (SIM and STORM). Since 2018 PLATIM accounts for a biophysics platform, equipped with 2 atomic force microscopes and a Triboindenter, for performing high resolution 3D topographies and/or for measuring mechanical properties (e.g. Young’s modulus) of isolated cells or biological tissues. A table top scanning electron microscope disposing of a cooling stage is also available. PLATIM is part of the regional core facility LYMIC, certified by the IBiSA label (IBiSA coordinates core facilities in the area of life sciences throughout France).
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Services
The Imaging and Microscopy Core Facility (PLATIM) provides scientists with a wide variety of services throughout the image acquisition process, including assistance in sample preparation. The users must attend a theoretical and practical training in order to have access to the instruments autonomously. Users may also be assisted in image analysis with customized data analysis programs, based on specifications established beforehand.
In addition, the core facility staff members actively monitor technological developments to provide optimal services to users and are active in initial and continuous training. They also participate in scientific outreach programs.
Our goals:
- Supporting research activities
- Providing imaging systems
- Providing training and assistance
- Participating to academic teaching and scientific outreach programs
- Maintaining a technological watch
Equipments
Our platform provides the following equipment:
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- Bioscope II Catalyst (Bruker).
- Nanowizard III (JPK).
- Triboindenter TI 950 Hysitron (now Bruker).
Microscopie super-résolution
Confocal microscopy
- Inverted confocal microscope AxioObserverZ1 LSM800 (Zeiss).
- Upright spectral confocal microscope SP5 (Leica).
- Upright spectral confocal microscope SP8 (Leica) with resonant scanning.
- Inverted confocal microscope LSM710 (Zeiss) coupled with a two-photons laser (Coherent).
- 2 upright confocal microscopes LSM700 (Zeiss) for observation of large samples.
- Inverted spinning disk confocal (Leica, Yokogawa) with FRAP/Photo-cativation module.
High-throughput confocal microscopy (High Content Screening)
- Inverted spinning disk confocal (Yokogawa), integrated analysis software
Conventional microscopy
- Upright microscope AxioImagerZ1 (Zeiss), with 2 cameras (color and monochrome).
- Inverted microscope Timelapse Axio Observer Z1 (Zeiss), with 2 monochrome cameras (sCMOS and EMCCD), temperature and CO2 control system.
- Inverted microscope Axiovert 135 (Zeiss), with a monochrome camera.
- Upright microscope Axiophot (Zeiss), with a monochrome camera (Lyon Sud).
- Upright microscope AxioImager Z1 (Zeiss), with a monochrome camera (Lyon Sud).
Macroscopy
- Stereomicroscope LUMAR (Zeiss) with epifluorescence, equipped with a monochrome camera
- Macroscope Leica Macrofluo Z16 APA A (Leica) with epifluorescence, equipped with a monochrome camera.
Electron microscopy
- Benchtop scanning electron microscope SH-3000 (Hirox)
Image analysis
- Two dedicated workstations.
In development
- Implementation of a “facility-made” light sheet, based on the openSPIM setup.
- Development of a pressure probe for measuring turgor pressure of plant cells (IMPRESS and Turgomap projets).
How to contact us/where to find us:
The Imaging and Microscopy Core Facility is located at the École Normale Supérieure de Lyon and at the University Hospital Lyon Sud. Instruments can be booked on-line by all the users having received the training and applied for an individual user account.
For more information, please contact us at:
- Mail : platim-ifr@ens-lyon.fr
- Tel : 04 72 72 87 62
- Head of the core facility: Jacques Brocard
Training
The training on the instruments is mandatory. This training is free of charges for academic users.
Staff members provide various types of trainings and courses: to 2nd year Master’s students, continuous trainings (workshops on microscopy CNRS, INSERM, INRA,…) and they participate to scientific outreach programs (Annual Science Festival, activities for high school students (French or foreign students…)).
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Documents
Links
Microscopy:
- Optical Microscopy Primer : educational website on optical microscopy.
- Florescence spectraviewer ThermoFisher
- Fpbase fluorescent protein database
- GDR ImaBio " Regard sur le vivant"
- RTmfm : Réseau Technologique de Microscopie photonique de Fluorescence Multidimensionnelle: french network on fluorescence microscopy.
- Réseau microscopie à sonde locale: french network on scanning probe microscopies.
Our softwares:
- Metamorph (Molecular Devices)
- Zeiss
- Huygens
- Imaris
- ImageJ
- Data Processing (JPK)
Nanoscope Analysis (Bruker)
