PRESENTATION

X-ray crystallography applied to proteins allows for the resolution of their three-dimensional structure at high resolution. Thanks to robotic equipment, you can screen several hundred crystallisation conditions with approximately 2 mg of protein. The Mosquito pipetting robot (STPLabtech) produces nanodrops with hundreds of different crystallisation solutions supplied by PSF. The RockImager (Formulatrix) and XtalFocus (ExploraNova) plate viewing robots allow for the thermoregulated storage of crystallisation plates at 19°C or 4°C as well as their secure, automatic remote viewing.

SERVICES

• Provision of consumables and use of equipment upon training: accessible to crystallography teams
• Collaborative structural crystallography study project: from protein production to 3D structure resolution, in collaboration with a crystallographer.
• Protein characterisation service using different biophysical approaches: DLS (homogeneity, aggregation), SEC-MALS (MW and oligomerisation), Circular Dichroism (folding and secondary structure content)
• Molecular interaction studies using interferometry (BLITZ Pall ForteBio) and Thermal Shift Assay (QPCR StepOne Thermofisher).

In partnership with UMR5086 MMSB, we can also offer thermophoresis (Monolith NT115 - Nanotemper), NanoDSF (Prometheus NT48 - Nanotemper) or microcalorimetry (ITC200).

Please note  : Analyses on our Biacore T200 are no longer carried out by PSF as a service . Contact us if you are already trained and would like to access the instrument, or if you would like to start a collaborative project

EQUIPMENT

Mosquito (STPLabtech)
RockImager 182 (Formulatrix)
XtalFocus (ExploraNova)
SEC-MALS MiniDawn Treos, TrEx (Wyatt)
1 DLS Nanosizer Diffusion Light Scattering (Malvern)
Dichroïsme circulaire Chirascan (Applied Photophysics)
BLITZ Pall ForteBio