AniRA Mouse models
Purpose
The Experimental mouse models core facility generates and produces genetically modified mice as well as infectious models analysis. This core facility provides a wide variety of services and constantly develops new tools to generate and validate experimental models.
The AniRA-Experimental mouse models facility has an animal core facility (Anira-PBES) that includes a state-of-the-art unit for transgenesis by lentiviral vectors, among others, developed with the genetic analysis core facility (AniRA-Genetic Analysis) and the vectorology core facility (Anira-Vectorology). The Anira-PBES animal facility has protected areas for experiments that require A1, A2 and A3 containment for proper handling of infectious models. The facility also has a L3 laboratory (AniRA-BSL3) that provides the scientific community with infectious animal models.
SERVICES
PRODUCTION OF VIRAL VECTORS
Production of lentiviral vectors for stable and effective transfer of nucleic sequences (gene, cDNA, Sh/miRNA) in cell lines, primary cells that are difficult to transfect (stem cells...) or in a whole organism.
The core facility provides expertise on:
- Selection of vectors, for the cloning of the genes of interest and for assigning the vectors to the appropriate biosafety level.
- Production of vectors overexpressing endogenous genes or for the ectopic expression of a gene in a cell line.
- Production of vectors for gene knock-out (KO) coding shRNAs against the RNA of the gene of interest.
- Production of vectors regulating the expression of genes or conditioning the KO of the gene expression by inducible lentivectors.
- Production of C2 or C3 classified vectors
- Assays based on the expression of a reporter protein, or by selection with an antibiotic or by Q-PCR.
More information : AniRA-Vectorology
GENERATION OF MUTANT LINES
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Production of genetically modified lines by:
- Traditional transgenesis (DNA, BAC),
- Homologous recombination in stem cells,
- Lentigenesis,
- Genomic engineering (TALE nuclease and CRISPR/Cas9).
More information : AniRA-PBES
DECONTAMINATION OF LINES AND CRYOPRESERVATION
- Decontamination by aseptic embryo transfer.
- Cryopreservation and distribution of embryos, sperm and ovaries.
More information : AniRA-PBES
GENOTYPING
- Routine genotyping of mutant animals by PCR.
- To determine the number of copies of a transgene by qPCR
- qPCR technologies
More information : AniRA-PBES
BREEDING AND PRODUCTION OF MURINE LINES
- Breeding and production of SPF (specified pathogen free) mice in ventilated cages => 3000 cages.
- Housing of mice in experimental facilities classified L1, L2 and L3 (2000 cages)
More information : AniRA-PBES
MOUSE INFECTIOUS MODELS
Production of models in facilities classified A2, A3 or BSL2 and BSL3:
- Development of new models of infectious diseases
- Development of humanized murine models to study human pathogens
- Interactions between new viruses and their vector (mosquito) and host (animal, human)
More information onAniRA-BSL3 and AniRA-PBES
QUALITY MANAGEMENT
To respond more effectively to the needs of their users and also, to ensure transparency and reliability of research results, the Management of the SFR BioSciences is committed to implement an ISO 9001 quality policy. To this end, it has created a Quality Management Department. This department actively coordinates the implementation of the quality projects of the technical core facilities of the SFR, especially those of the regional AniRA facility certified with the IBiSA label.
More information on Quality managment
ANIRA R&D PROGRAM
Since 2014, SFR Biosciences organizes a yearly call for proposals to support innovative and collaborative projects of methodological or technological development. Projects are evaluated by a scientific committee that consists of four researchers and four staff members of the core facilities. Projects are evaluated based on their scientific quality, innovative nature, value for the scientific community, and potential new services for the SFR Biosciences.
AniRA R&D project supported in 2017
- Insertion ciblée de gros fragments (Editing souris)
AniRA R&D projects supported in 2016
- Recombinaison homologue par CRISPR/Cas9 chez la souris
- Livraison non intrusive de matériel génétique dans l’ovocyte de souris
AniRA R&D projects supported in 2015
- Zisupton : création d'un nouveau vecteur à l'aide d'un transposon
AniRA R&D projects supported in 2014
- Création de séries alléliques par CRISPR/Cas9 chez la souris