AniRA Mouse models
PRESENTATION
The Mouse Models department is dedicated to the generation and production of genetically modified mice, as well as the development and use of infectious animal models. This department offers a wide range of services and continuously develops new tools for the generation and validation of experimental models.
AniRA Mouse Models operates a central animal facility (AniRA-PBES) that includes a high-performance transgenesis service. This service makes use of lentiviral vectors developed in collaboration with the Genetic Analysis platform (AniRA-Analyse Génétique) and the Vectorology platform (AniRA-Vectorology).
The AniRA-PBES animal facility includes A1, A2, and A3 biosafety areas, enabling the use of infectious models. In addition, the department operates a BSL3 laboratory (AniRA-BSL3), providing the scientific community with access to relevant infectious animal models.
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SERVICES OFFERED
Viral Vector Production
Production of lentiviral vectors for the stable and efficient transfer of nucleic acid sequences (gene, cDNA, sh/miRNA) into cell lines, hard-to-transfect primary cells (e.g. stem cells), or whole organisms.
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Guidance in vector choice, cloning of genes of interest, and biosafety requirements.
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Production of vectors for endogenous gene overexpression or ectopic gene expression in cell lines.
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Production of vectors for gene knock-out using shRNA-coding vectors targeting the RNA of the gene of interest.
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Production of vectors for gene expression regulation or conditional knock-out using inducible lentivectors.
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Vector production under biosafety levels C2 or C3.
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Vector titration based on reporter protein expression, antibiotic selection, and qPCR analysis.
For more info: AniRA-Vectorologie
Mutant Line generation
Production of genetically modified mouse lines through:
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Classical transgenesis (DNA, BAC),
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Homologous recombination via ES cells,
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Lentigenesis,
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Genome engineering (TALEN, CRISPR/Cas9).
For more info: AniRA-PBES
Line Decontamination and Cryopreservation
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Line decontamination by aseptic embryo transfer.
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Cryopreservation of lines as embryos, sperm, or ovaries, with storage and distribution services.
For more info: AniRA-PBES
Genotyping
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Routine PCR-based genotyping of mutant animals.
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Determination of transgene copy number by qPCR.
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Advanced qPCR technologies.
For more info: AniRA-Analyse Génétique
Breeding and Line Production
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Breeding and production of murine lines in ventilated cages under EOPS (Exempt of Specific Pathogen Organisms) conditions → up to 3,000 cages.
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Housing and maintenance of animals in A1, A2, or A3 experimental biosafety areas → up to 2,000 cages.
For more info: AniRA-PBES
Infectious Models
Production of models under A2, A3, BSL2, and BSL3 conditions:
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Development of new infectious disease models.
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Development of humanized mouse models for the study of human pathogens.
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Studies of interactions between emerging viruses, their vectors (e.g. mosquitoes), and their hosts (animals, humans).
For more details: AniRA-BSL3 and AniRA-PBES
PROGRAM R&D "ANIRA "
Since 2014, SFR Biosciences has organized an annual call for proposals to support collaborative projects focused on methodological or technological innovation. Projects are evaluated by a scientific committee composed of four researchers and four platform staff members.
Evaluation criteria include:
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Scientific quality and innovative character,
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Interest for the scientific community,
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Potential for developing new services within the SFR Biosciences platforms.
QUALITY MANAGEMENT
To better meet users’ needs and ensure both transparency and reliability of research outcomes, the management of SFR Biosciences initiated an ISO 9001 quality process and created a dedicated Quality Management Unit.
This unit actively coordinates the operational implementation of quality projects across the technical platforms of SFR, including AniRA, which has been IBiSA-labeled.
