AniRA Phenotyping
Purpose
The AniRA-Phenotyping Unit provides comprehensive phenotyping of different physiological systems: cardiovascular, metabolism, immune system and bone system. Phenotyping enables standard and functional characterization in mice. The AniRA-Phenotyping Unit aims to better understand how genes function, the basic biological mechanisms, infectious pathophysiology to identify and validate new therapeutic targets, for instance.
The AniRA-Phenotyping Unit provides investigators with customized phenotyping services:
- Phenotyping the immune and bone system (AniRA-ImmOs) largely through the core facilities of flow cytometry AniRA-Cytométrie and animal facility AniRA-PBES.
- Phenotyping in the fields of metabolism, cardiovascular system and autonomic nervous system (AniPhy)
- Studies on neurotransmitters (NeuroChem) using biosensors for the assay of small molecules customized in the core facility.
SERVICES
Immune system
The AniRA ImmOs core facility uses standardized, quantitative and validated methods to analyze the immune system.
AniRA ImmOs provides:
- Phenotypic analyses of the immune system in genetically modified mice
- Validated physiopathological models to evaluate the immune system in mice
- Innovative multiparametric tests
- Expertise and technical support
The activity is mainly carried out in the flow cytometry facility AniRA-Cytométrie and the animal facility AniRA-PBES. Both facilities have a large range of equipment.
Bone system
AniRA ImmOs provides state-of-the-art equipment and services, entirely dedicated to the study of mineralized tissues. AniRA ImmOs can characterize the components of the bone system. The methods used have become standard and are particularly appropriate to diagnose and study models such as osteoporosis or osteopetrosis.
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Two types of services are available:
- Equipment and training
- Customized investigations from receipt of samples to result analysis.
metabolism
The AniPhy core facility offers expertise and assistance through specific investigations (upon request) and complete metabolic analyses to evaluate in vivo the energy balance in mice. These phenotyping studies are performed under basal conditions or modified energies using standard techniques for detecting defects in energy metabolism, homeostasis of glucose etc. …. The AniPhy core facility also provides technical and scientific support to design experimental protocols and develop new techniques for metabolism studies.
The AniPhy core facility is located on the campus Rockefeller (SFR Health) and is part of the network of technical facilities and services of the University Claude Bernard de Lyon.
Cardiovascular system and autonomic nervous system
The AniPhy core facility evaluates in vivo the cardiovascular function in rats and mice to analyze the main cardiac phenotypes and heart diseases such as arrhythmias, hypertension and hypotension. AniRA-AniPhy is located on the campus Rockefeller (SFR Health) and is part of the network of core facilities and shared services of the University Claude Bernard de Lyon.
Neurochemistry
The NeuroChem core facility provides the scientific community, the opportunity to perform biochemical analyses of the whole nervous system or neuronal tissues with microsensors. NeuroChem provides tools not only to the IFNL (Institut Fédératif des Neurosciences Lyon) but also to external research teams, hospitals, and the pharmaceutical industry together with customized services and training.
QUALITY MANAGEMENT
To respond more effectively to the needs of their users and also, to ensure transparency and reliability of research results, the Management of the SFR BioSciences is committed to implement an ISO 9001 quality policy. To this end, it has created a Quality Management Department. This department actively coordinates the implementation of the quality projects of the technical core facilities of the SFR, especially those of the regional AniRA facility certified with the IBiSA label.
For more information on Quality Management
ANIRA R&D PROGRAM
Since 2014, SFR Biosciences organizes a yearly call for proposals to support innovative and collaborative projects of methodological or technological development. Projects are evaluated by a scientific committee that consists of four researchers and four staff members of the core facilities. Projects are evaluated based on their scientific quality, innovative nature, value for the scientific community, and potential new services for the SFR Biosciences.
AniRA R&D projects supported in 2015
- Apport de la cytométrie de masse pour le diagnostic et la réponse au traitement du rejet infraclinique en transplantation rénale
AniRA R&D projects supported in 2014
- Optimisation d’une analyse quantitative et qualitative de l’Autophagie par ImageStreamX