PRECI
Purpose
The PRECI core facility (Plateau de Recherche Expérimentale en Criblage In Vivo) is a high-throughput screening facility that houses aquatic models (zebrafish, platyfish, Japanese medaka,...). Procedures such as injections, behavioral tests with larvae can also be performed here.
The main activities of PRECI are:
- To breed aquatic models
- To develop the technological know-how required to generate transgenic lines
- To receive, breed and store transgenic lines (including quality control).
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Services
The PRECI core facility harbors various aquatic models required for research projects. It is possible for PRECI to house different species. Currently it has zebrafish, Japanese medakas, platyfish.
The facility preserves the founders, breeds a large number of animals for experiments and collects a daily production of eggs for the zebrafish and medaka models.
Applications for access to the services must be sent by e-mail to: PRECI.Lyon@inserm.fr.
A scientific committee evaluates the feasibility of the proposals. Experimental protocols performed in the facility must be approved beforehand by CECCAPP. Every new user must be trained. After training, users must sign the facility’s Internal Rules regarding access and working procedures. Users who fail to observe the working procedures of the facility may be denied access.
Equipment
The PRECI facility has customizable tank facilities including a Tecniplast system equipped with an automated Tritone feeding system (up to 20,000 fish) and custom-designed LuxAqua systems (more than 2,000 fish). The facility also houses one experimental laboratory, with the necessary equipment to establish transgenic lines.
- [Site de réservation du micro-injecteur]
- Zone d’élevage :
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- Système de traitement de l’eau : osmoseur, adoucisseur (SFEC) pour animaux d’eau douce .
- Système d’hébergement (Tecniplast et Luxaqua) : capacité de 20 000 ovipares et 2 000 ovovivipares
- Système de nourrissage automatique (Tecniplast)
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- Zone expérimentale :
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- 4 Micro Injecteurs (Eppendorf FemtoJet) - 1 Stéréomicroscope (SZX2-ZB10, Olympus).
- 1 Etireuse verticale (PC10, Olympus).
- 1 Enceinte pour tests environnementaux (MLR-351SANYO, Dutscher).
-1 Platine éclairante 5’’ (127x127 mm Objectif Planapo 0.5xBague, monture C Filtre bleu en monture, Leica).
- 1 système d'analyse de comportement des larves (Viewpoint).
- 1 microscope fluo Leïca / caméra et son PC (prêtés par Jean-Nicolas Volff)
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How to contact us/where to find us:
The PRECI facilities are located in IGFL site.
- IGFL, 32-34 avenue Tony Garnier 69007 Lyon
For further information, please contatc :
- Bernard Laure: Facility's manager
- Delphine Galiana : Scientific Coordinator
- Tel : 04 26 73 13 24
- Mail : preci.lyon@inserm.fr
Training
Training in Animal Experiments, Level 1: "Conventional and transgenic animal models for biological and medical research – Animal Facility Technology”, Inter-University Degree (I.U.D.), École Normale Supérieure de Lyon/Université Claude Bernard Lyon I - 1 session per year.