3- Protein production and peptide synthesis
PRESENTATION
The biochemistry platform allows the production of pure and homogeneous proteins, for functional or structural studies. Recombinant protein expression is performed in a bacterial system only. Purification steps are carried out by chromatography, and various quality controls are performed to finalise the production. Experiments can be performed by the facility staff or by the user themselves.
The centre also offers a peptide synthesis service and an automated immunoassay service on the JESS system.
SERVICES
Different levels of services are offered: full services or independent use of equipment upon training
1. Full support for a protein production project
| From the recombinant plasmid, expression and solubility control, expression in bacterial system, purification by chromatography or other, dosages and quality control by TSA, NanoDSF or on biophysical equipment of circular dichroism, SEC-MALS and DLS |  |
2. Peptide Synthesis Service
Synthesis of up to 70 residues with a wide choice of scales from 0.05 to 5 mmol, with different post-translational modifications (biotinylation, chromophore, 13C and or 15N labeling, cyclisation, etc.). In the case of small proteins, peptide synthesis can be an alternative for the production of around ten mg, at a lower cost.
3. Immunoassay Service on JESS
| A sensitive, reproducible and quantitative immunoassay in 3 hours! This is a capillary migration system (cartridges of 25 capillaries) for separation according to 3 ranges (2-40 kDa, 12-230 kDa, or 66-440 kDa) with chemiluminescence or fluorescence detection. Possibility of quantification, multiplexing in the same capillary. The sensitivity is much better than in classic western blot (3 µg of proteins). More than 3000 primary antibodies are validated by the system, allowing them to be used in optimal conditions. |  |
4. Provision of biochemistry equipment
Mandatory training for the use of equipment, provision of consumables. Agreement is required before independent use.
EQUIPMENT
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